Fungicide for leyland cypress needle blight11/25/2023 A further constraining factor is that only a small number of dematiaceous hyphomycetes have been included in genetic phylogenies using DNA loci, mRNA and proteins. Proper identification of these organisms is further complicated by the numerous name revisions over the last two decades. sequoiae populations is that numerous dematiaceous hyphomycetes with morphologically similar conidia and conidioma are found in many regions (Figs. in GenBank (NCBI), corresponding to the 18S rDNA gene of this fungus, a total of 5476 base pairs (bp).Ī problem in sampling P. Currently, only three entries are listed for Passalora spp. A lack of genetic information for this fungus prevents utilization of genetic tools to determine genetic diversity of isolates, potential differences in virulence, and ultimately the development of control practices. sequoiae using PacBio and Illumina to assemble contigs. The objective of this work was to sequence the whole genome of P. Mapping of Illumina reads to PacBio contigs resulted in a 1000 × coverage and were used to confirm accuracy of the consensus sequences. Based on a total of 44 contigs with 722 kilobase (kb) average length (range 9.4 kb to 3.4 Mb), the whole genome size was estimated at 31,768,716 bp. Illumina reads were mapped to PacBio assembled contigs to determine base call consistency. The draft genome was obtained using Pacific Biosciences (PacBio) SMRT and Illumina HiSeq 2500 sequencing. sequoiae 9LC2 that was isolated from Leyland cypress ‘Leighton Green’ in 2017 in southern Mississippi, USA. sequoiae as a resource for primer development to investigate genotype diversity. The objective was to generate a high-quality draft assembly of the genome of P. Passalora sequoiae (family Mycosphaerellaceae) causes a twig blight on Leyland cypress that requires numerous fungicide applications annually to minimize economic losses for ornamental plant nursery and Christmas tree producers.
0 Comments
Leave a Reply.AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |